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iodixanol step gradient optiprep (Cosmo Bio USA)

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Cosmo Bio USA iodixanol step gradient optiprep
Iodixanol Step Gradient Optiprep, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optiprep+step+gradient/density+gradient+axs+1114542/pmc11126825-321-20-29
Average 90 stars, based on 1 article reviews

iodixanol step gradient optiprep - by Bioz Stars, 2026-11

90/100 stars

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Bioprocessing:

Article Title: Noradrenergic efferent subsystems that gate traumatic social learning
Article Snippet: Cells were cultured in Dulbeccos modified Eagles medium with high glucose and GlutaMAX supplement (DMEM, high glucose, GlutaMAX Supplement; 10566024, Thermo Fisher Scientific, Waltham, MA) containing 10% fetal bovine serum (Sigma-Aldrich, St Louis, MO) at 37°C with 5% CO 2 . .. The AAV vectors were collected from the cells and media by ultracentrifugation using an iodixanol step gradient (15%, 25%, 40%, and 60%; Optiprep, 1893, Cosmo Bio, Tokyo, Japan) and were replaced and concentrated with phosphate-buffered saline (PBS) containing 0.001% (v/v) Pluronic F-68 (24040032, Thermo Fisher Scientific) via ultrafiltration using an Amicon Ultra-15 centrifugal filter (100-kDa cutoff; UFC910024, Millipore). .. The viral titers were quantified by a quantitative real-time PCR using GoTaq qPCR Master Mix (Cat# A6001, Promega, Madison, WI), with a linearized AAV genome plasmid serving as a standard.

Saline:

Suspension:

Article Title: Optimization of AAV vectors for transactivator-regulated enhanced gene expression within targeted neuronal populations
Article Snippet: The cell suspension was subjected to three freeze-thaw cycles and treated with ≥250 U/μL of Benzonase nuclease (Sigma-Aldrich, St Louis, MO) at 37°C for at least 40 min. .. The suspension was centrifuged at 3,000 × g for 15 min at 4°C, and the supernatant was loaded onto an iodixanol step gradient (15, 25, 40, and 60%; Optiprep, Cosmo Bio) and centrifuged at 278,400 × g for 105 min at 18°C. .. After centrifugation, the 40/60% interface and the 40% layer containing AAV vectors were collected and replaced with D-PBS containing 0.001% (v/v) Pluronic F-68 via ultrafiltration using an Amicon Ultra-15 centrifugal filter (100-kDa cutoff; UFC910024, Millipore) to concentrate the AAV vectors.

Article Title: Claustrum mediates bidirectional and reversible control of stress-induced anxiety responses.
Article Snippet: .. The suspension was centrifuged at 3000g for 15 min at 4°C, and the supernatant was loaded onto an iodixanol step gradient (15, 25, 40, and 58%; Optiprep, Cosmo Bio, Tokyo, Japan) and centrifuged at 278,400g for 105 min at 18°C. ..

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$Isolation of microglia from adult rat mesencephalon using density gradient fractionation. (A) Cellular pool from the midbrain of 10-week-old Wistar rat using OptiPrep step gradient separation method. The fraction and cellular designation were based on the previous study . Note the pellet at the bottom that contains microglia. (B) Double immunocytofluorescence labeling of Hoechst (nucleus) and one of the microglial (OX42), astrocytic (GFAP), and neuronal (NeuN) markers using primary cultured cells obtained from the pellet in (A) . Scale bar represents 100 μm. (C) Semi-quantification of the number of OX42-ir, GFAP-ir, and NeuN-ir cells against Hoechst positive nuclei. The results are expressed as the percentage of the number of OX42-ir, GFAP-ir, and NeuN-ir cells to the total number of Hoechst-ir nuclei. * P < 0.05 compared with value from OX42-ir result (ANOVA with post hoc Student’s t test). (D) Immunocytofluorescence of OX6 and iNOS in primary cultured microglia originated from the contralateral (Cont) or ipsilateral (Ipsi) SN at 7 dpl. Microglia cultivated from the ipsilateral SN are highly immunoreactive with OX6 and iNOS, meaning that they are highly activated and produce high level of reactive nitrogen species. Scale bar represents 20 μm.$

Journal: BMC Neuroscience

Article Title: Down-regulation of microglial activity attenuates axotomized nigral dopaminergic neuronal cell loss

doi: 10.1186/1471-2202-14-112

Figure Lengend Snippet: Isolation of microglia from adult rat mesencephalon using density gradient fractionation. (A) Cellular pool from the midbrain of 10-week-old Wistar rat using OptiPrep step gradient separation method. The fraction and cellular designation were based on the previous study . Note the pellet at the bottom that contains microglia. (B) Double immunocytofluorescence labeling of Hoechst (nucleus) and one of the microglial (OX42), astrocytic (GFAP), and neuronal (NeuN) markers using primary cultured cells obtained from the pellet in (A) . Scale bar represents 100 μm. (C) Semi-quantification of the number of OX42-ir, GFAP-ir, and NeuN-ir cells against Hoechst positive nuclei. The results are expressed as the percentage of the number of OX42-ir, GFAP-ir, and NeuN-ir cells to the total number of Hoechst-ir nuclei. * P < 0.05 compared with value from OX42-ir result (ANOVA with post hoc Student’s t test). (D) Immunocytofluorescence of OX6 and iNOS in primary cultured microglia originated from the contralateral (Cont) or ipsilateral (Ipsi) SN at 7 dpl. Microglia cultivated from the ipsilateral SN are highly immunoreactive with OX6 and iNOS, meaning that they are highly activated and produce high level of reactive nitrogen species. Scale bar represents 20 μm.

Article Snippet: The collected supernatant was loaded upon the top of the prepared OptiPrep step gradient (Sigma-Aldrich Co., St. Louis, USA) solution.

Techniques: Isolation, Fractionation, Labeling, Cell Culture